【文獻標題】Excessive ammonia inhalation causes liver damage and dysfunction by altering gene networks associated with oxidative stress and immune function

【作者】Xiangyin Zeng , Runze Liu , Yutao Li , et.al

【作者單位】東北農(nóng)業(yè)大學(xué)（Northeast Agricultural University）

【文獻中引用產(chǎn)品】

豬白介素4(IL-4)ELISA試劑盒

豬白介素6(IL-6)ELISA試劑盒

豬白介素8(IL-8)ELISA試劑盒

豬免疫球蛋白G(IgG)ELISA試劑盒

豬免疫球蛋白M(IgM)ELISA試劑盒

【關(guān)鍵詞】Ammonia ,Liver damage ,Oxidative stress ,Immune dysfunction ,Gene network

【DOI】doi.org/10.1016/j.ecoenv.2021.112203

【影響因子(IF)】6.2

【出版期刊】《Ecotoxicology and Environmental Safety》

【產(chǎn)品原文引用】

2.5. Measurement of immune function 

To assess the effect of ammonia exposure on immune function, serum samples were used to analyze cytokine and immunoglobulin levels at Northeast Agricultural University. The cytokines (IL-4, IL-6 and IL-8) were determined by ELISA kits according to the kit instructions (Shanghai Hengyuan Biotechnology Co., Ltd, China). All cytokines were reported in ng/L. Inter and intra assay cytokine CVs were IL-4 (14.67%;< 16%), IL-6 (10.12%; < 13%) and IL-8 (13.1%;< 16%). Serum immunoglobulin-A (IgA), IgG and IgM were detected by ELISA kits using the enzyme-labeled instrument (SpectraMax? ABS00254, California, USA). All immunoglobulin levels were expressed in mg/mL. IgA ELISA kit was purchased from ShangHai Yanjin Biotechnology Co., Ltd (China). IgG and IgM ELISA kits were detected by ELISA kits (Shanghai Hengyuan Biotechnology Co., Ltd, China). 

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